mouse monoclonal αv5 Search Results


mouse monoclonal αv5  (Bio-Rad)
96
Bio-Rad mouse monoclonal αv5
(A) Tethering assay. FBF-2 is tethered by λN22 binding to boxB hairpins in the reporter RNA. Modified from Aoki et al. 201891. (B) Above, location of gonadal arm (black box) within animal. Below, location of distal region (dotted box) within gonadal arm. Asterisk marks distal end. Germline stem cells (GSCs) reside at the most distal end of germline and their daughters begin differentiation as they move proximally. (C) Diagram of distal gonad, showing distance from the distal end in microns below and extents of abundant FBF-2 (black line) and lower FBF-2 (dotted line) above. (D-E) Representative z-projections of extruded germlines. 20μm scale bar in (D) applies to all images. Dotted line marks gonad boundary; asterisk marks distal end. Top, GFP reporter expression (green); bottom, FBF-2FLAG staining (magenta). (D) Untethered wild-type FBF-2. (E) Tethered wild-type FBF-2. (F) ImageJ quantitation of reporter signal from tethered vs untethered FBF-2. GFP abundance plotted against distance from distal end. Solid line shows mean abundance and shading shows 95% confidence interval. Each plot represents three biological replicates with at least 10 gonad arms per replicate. P-values are given for pooled data in 0-35, 35-70 and 70-100 μm regions (black bars). P-values: *** p < 0.001, ** p < 0.01, * p < 0.05, ns (not significant) p > 0.05. Exact p-values in Table S4. (G-H) Representative z-projections of extruded germlines, as in (3D-E). (G) Untethered Y479A. (H) Tethered Y479A. (I) Quantitation of tethered vs untethered Y479A. P-values as in (F). (J) Western blots after FBF-2 and NTL-1 co-immunoprecipitation. Left, input lysates (1%); right, FLAG IP (10%). NTL-1:V5 co-immunoprecipitates with both wild-type and Y479A.
Mouse Monoclonal αv5, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+monoclonal+%CE%B1v5/Mouse+anti+V5-Tag/pmc11253550-3-0-4
Average 96 stars, based on 1 article reviews
mouse monoclonal αv5 - by Bioz Stars, 2026-09
96/100 stars
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vinculin antibody  (NSJ Bioreagents)
99
NSJ Bioreagents vinculin antibody
(A) Tethering assay. FBF-2 is tethered by λN22 binding to boxB hairpins in the reporter RNA. Modified from Aoki et al. 201891. (B) Above, location of gonadal arm (black box) within animal. Below, location of distal region (dotted box) within gonadal arm. Asterisk marks distal end. Germline stem cells (GSCs) reside at the most distal end of germline and their daughters begin differentiation as they move proximally. (C) Diagram of distal gonad, showing distance from the distal end in microns below and extents of abundant FBF-2 (black line) and lower FBF-2 (dotted line) above. (D-E) Representative z-projections of extruded germlines. 20μm scale bar in (D) applies to all images. Dotted line marks gonad boundary; asterisk marks distal end. Top, GFP reporter expression (green); bottom, FBF-2FLAG staining (magenta). (D) Untethered wild-type FBF-2. (E) Tethered wild-type FBF-2. (F) ImageJ quantitation of reporter signal from tethered vs untethered FBF-2. GFP abundance plotted against distance from distal end. Solid line shows mean abundance and shading shows 95% confidence interval. Each plot represents three biological replicates with at least 10 gonad arms per replicate. P-values are given for pooled data in 0-35, 35-70 and 70-100 μm regions (black bars). P-values: *** p < 0.001, ** p < 0.01, * p < 0.05, ns (not significant) p > 0.05. Exact p-values in Table S4. (G-H) Representative z-projections of extruded germlines, as in (3D-E). (G) Untethered Y479A. (H) Tethered Y479A. (I) Quantitation of tethered vs untethered Y479A. P-values as in (F). (J) Western blots after FBF-2 and NTL-1 co-immunoprecipitation. Left, input lysates (1%); right, FLAG IP (10%). NTL-1:V5 co-immunoprecipitates with both wild-type and Y479A.
Vinculin Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+monoclonal+%CE%B1v5/Vinculin+Antibody/custom%40r30091%4030111239
Average 99 stars, based on 1 article reviews
vinculin antibody - by Bioz Stars, 2026-09
99/100 stars
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anti-human endogenous retrovirus type k (herv k) envelope protein. igg fraction  (AMS Biotechnology)
96
AMS Biotechnology anti-human endogenous retrovirus type k (herv k) envelope protein. igg fraction
(A) Tethering assay. FBF-2 is tethered by λN22 binding to boxB hairpins in the reporter RNA. Modified from Aoki et al. 201891. (B) Above, location of gonadal arm (black box) within animal. Below, location of distal region (dotted box) within gonadal arm. Asterisk marks distal end. Germline stem cells (GSCs) reside at the most distal end of germline and their daughters begin differentiation as they move proximally. (C) Diagram of distal gonad, showing distance from the distal end in microns below and extents of abundant FBF-2 (black line) and lower FBF-2 (dotted line) above. (D-E) Representative z-projections of extruded germlines. 20μm scale bar in (D) applies to all images. Dotted line marks gonad boundary; asterisk marks distal end. Top, GFP reporter expression (green); bottom, FBF-2FLAG staining (magenta). (D) Untethered wild-type FBF-2. (E) Tethered wild-type FBF-2. (F) ImageJ quantitation of reporter signal from tethered vs untethered FBF-2. GFP abundance plotted against distance from distal end. Solid line shows mean abundance and shading shows 95% confidence interval. Each plot represents three biological replicates with at least 10 gonad arms per replicate. P-values are given for pooled data in 0-35, 35-70 and 70-100 μm regions (black bars). P-values: *** p < 0.001, ** p < 0.01, * p < 0.05, ns (not significant) p > 0.05. Exact p-values in Table S4. (G-H) Representative z-projections of extruded germlines, as in (3D-E). (G) Untethered Y479A. (H) Tethered Y479A. (I) Quantitation of tethered vs untethered Y479A. P-values as in (F). (J) Western blots after FBF-2 and NTL-1 co-immunoprecipitation. Left, input lysates (1%); right, FLAG IP (10%). NTL-1:V5 co-immunoprecipitates with both wild-type and Y479A.
Anti Human Endogenous Retrovirus Type K (Herv K) Envelope Protein. Igg Fraction, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+monoclonal+%CE%B1v5/Anti-human+Endogenous+Retrovirus+type+K+(HERV+K)+envelope+protein%2E+IgG+fraction/custom%40herm-1811-5%4019812154
Average 96 stars, based on 1 article reviews
anti-human endogenous retrovirus type k (herv k) envelope protein. igg fraction - by Bioz Stars, 2026-09
96/100 stars
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odyssey  (LI-COR)
99
LI-COR odyssey
(A) Tethering assay. FBF-2 is tethered by λN22 binding to boxB hairpins in the reporter RNA. Modified from Aoki et al. 201891. (B) Above, location of gonadal arm (black box) within animal. Below, location of distal region (dotted box) within gonadal arm. Asterisk marks distal end. Germline stem cells (GSCs) reside at the most distal end of germline and their daughters begin differentiation as they move proximally. (C) Diagram of distal gonad, showing distance from the distal end in microns below and extents of abundant FBF-2 (black line) and lower FBF-2 (dotted line) above. (D-E) Representative z-projections of extruded germlines. 20μm scale bar in (D) applies to all images. Dotted line marks gonad boundary; asterisk marks distal end. Top, GFP reporter expression (green); bottom, FBF-2FLAG staining (magenta). (D) Untethered wild-type FBF-2. (E) Tethered wild-type FBF-2. (F) ImageJ quantitation of reporter signal from tethered vs untethered FBF-2. GFP abundance plotted against distance from distal end. Solid line shows mean abundance and shading shows 95% confidence interval. Each plot represents three biological replicates with at least 10 gonad arms per replicate. P-values are given for pooled data in 0-35, 35-70 and 70-100 μm regions (black bars). P-values: *** p < 0.001, ** p < 0.01, * p < 0.05, ns (not significant) p > 0.05. Exact p-values in Table S4. (G-H) Representative z-projections of extruded germlines, as in (3D-E). (G) Untethered Y479A. (H) Tethered Y479A. (I) Quantitation of tethered vs untethered Y479A. P-values as in (F). (J) Western blots after FBF-2 and NTL-1 co-immunoprecipitation. Left, input lysates (1%); right, FLAG IP (10%). NTL-1:V5 co-immunoprecipitates with both wild-type and Y479A.
Odyssey, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+monoclonal+%CE%B1v5/Odyssey/custom%40Odyssey%4019812154
Average 99 stars, based on 1 article reviews
odyssey - by Bioz Stars, 2026-09
99/100 stars
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odyssey imaging system  (LI-COR)
99
LI-COR odyssey imaging system
(A) Tethering assay. FBF-2 is tethered by λN22 binding to boxB hairpins in the reporter RNA. Modified from Aoki et al. 201891. (B) Above, location of gonadal arm (black box) within animal. Below, location of distal region (dotted box) within gonadal arm. Asterisk marks distal end. Germline stem cells (GSCs) reside at the most distal end of germline and their daughters begin differentiation as they move proximally. (C) Diagram of distal gonad, showing distance from the distal end in microns below and extents of abundant FBF-2 (black line) and lower FBF-2 (dotted line) above. (D-E) Representative z-projections of extruded germlines. 20μm scale bar in (D) applies to all images. Dotted line marks gonad boundary; asterisk marks distal end. Top, GFP reporter expression (green); bottom, FBF-2FLAG staining (magenta). (D) Untethered wild-type FBF-2. (E) Tethered wild-type FBF-2. (F) ImageJ quantitation of reporter signal from tethered vs untethered FBF-2. GFP abundance plotted against distance from distal end. Solid line shows mean abundance and shading shows 95% confidence interval. Each plot represents three biological replicates with at least 10 gonad arms per replicate. P-values are given for pooled data in 0-35, 35-70 and 70-100 μm regions (black bars). P-values: *** p < 0.001, ** p < 0.01, * p < 0.05, ns (not significant) p > 0.05. Exact p-values in Table S4. (G-H) Representative z-projections of extruded germlines, as in (3D-E). (G) Untethered Y479A. (H) Tethered Y479A. (I) Quantitation of tethered vs untethered Y479A. P-values as in (F). (J) Western blots after FBF-2 and NTL-1 co-immunoprecipitation. Left, input lysates (1%); right, FLAG IP (10%). NTL-1:V5 co-immunoprecipitates with both wild-type and Y479A.
Odyssey Imaging System, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+monoclonal+%CE%B1v5/Odyssey+Imaging+System/custom%40odyssey-imaging-system%4019812154
Average 99 stars, based on 1 article reviews
odyssey imaging system - by Bioz Stars, 2026-09
99/100 stars
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n a mouse monoclonal αph3 cell signaling technology  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc n a mouse monoclonal αph3 cell signaling technology
(A) FBF-1 and FBF-2 are redundant for three germline roles. GSC, germline stem cell. (B) Adult gonad organization. Progenitor Zone (PZ, yellow) includes a pool of GSCs adjacent to the distal end (asterisk) and GSC daughters making the sperm to oocyte cell fate switch (s/o switch) more proximally; mature sperm (green) are in spermatheca and oocytes develop in proximal arm (magenta). The distal end is capped by the stem cell niche. (C-F) Representative z-projection images of extruded adult gonads; stained for sperm (α-SP56, green), oocytes (α-RME-2, magenta) and DNA (DAPI, cyan). Dotted line marks gonad boundary; asterisk marks distal end; yellow line marks PZ extent. 20μm scale bar in (C) applies to (C-F) (G) Gonad stained by smFISH for lst-1 RNA, a GSC marker (yellow). 20μm scale bar in (G) applies to (G-H). (H) Gonad stained with α-phospho-histone H3 (PH3) to visualize M-phase chromosomes (yellow) and DAPI (cyan). (I) Y479A-only germline phenotype. GSCs, germline stem cells maintained; s/o switch, sperm to oocyte switch occurs, n, number gonads scored. See also Figure S6.
N A Mouse Monoclonal αph3 Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+monoclonal+%CE%B1v5/EGR1+Rabbit+mAb/pmc11253550-623-63-67
Average 93 stars, based on 1 article reviews
n a mouse monoclonal αph3 cell signaling technology - by Bioz Stars, 2026-09
93/100 stars
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rb α zc3hav1  (Proteintech)
93
Proteintech rb α zc3hav1
(A) FBF-1 and FBF-2 are redundant for three germline roles. GSC, germline stem cell. (B) Adult gonad organization. Progenitor Zone (PZ, yellow) includes a pool of GSCs adjacent to the distal end (asterisk) and GSC daughters making the sperm to oocyte cell fate switch (s/o switch) more proximally; mature sperm (green) are in spermatheca and oocytes develop in proximal arm (magenta). The distal end is capped by the stem cell niche. (C-F) Representative z-projection images of extruded adult gonads; stained for sperm (α-SP56, green), oocytes (α-RME-2, magenta) and DNA (DAPI, cyan). Dotted line marks gonad boundary; asterisk marks distal end; yellow line marks PZ extent. 20μm scale bar in (C) applies to (C-F) (G) Gonad stained by smFISH for lst-1 RNA, a GSC marker (yellow). 20μm scale bar in (G) applies to (G-H). (H) Gonad stained with α-phospho-histone H3 (PH3) to visualize M-phase chromosomes (yellow) and DAPI (cyan). (I) Y479A-only germline phenotype. GSCs, germline stem cells maintained; s/o switch, sperm to oocyte switch occurs, n, number gonads scored. See also Figure S6.
Rb α Zc3hav1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+monoclonal+%CE%B1v5/ZC3HAV1+Antibody/pm37405998-264-73-77
Average 93 stars, based on 1 article reviews
rb α zc3hav1 - by Bioz Stars, 2026-09
93/100 stars
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Image Search Results


(A) Tethering assay. FBF-2 is tethered by λN22 binding to boxB hairpins in the reporter RNA. Modified from Aoki et al. 201891. (B) Above, location of gonadal arm (black box) within animal. Below, location of distal region (dotted box) within gonadal arm. Asterisk marks distal end. Germline stem cells (GSCs) reside at the most distal end of germline and their daughters begin differentiation as they move proximally. (C) Diagram of distal gonad, showing distance from the distal end in microns below and extents of abundant FBF-2 (black line) and lower FBF-2 (dotted line) above. (D-E) Representative z-projections of extruded germlines. 20μm scale bar in (D) applies to all images. Dotted line marks gonad boundary; asterisk marks distal end. Top, GFP reporter expression (green); bottom, FBF-2FLAG staining (magenta). (D) Untethered wild-type FBF-2. (E) Tethered wild-type FBF-2. (F) ImageJ quantitation of reporter signal from tethered vs untethered FBF-2. GFP abundance plotted against distance from distal end. Solid line shows mean abundance and shading shows 95% confidence interval. Each plot represents three biological replicates with at least 10 gonad arms per replicate. P-values are given for pooled data in 0-35, 35-70 and 70-100 μm regions (black bars). P-values: *** p < 0.001, ** p < 0.01, * p < 0.05, ns (not significant) p > 0.05. Exact p-values in Table S4. (G-H) Representative z-projections of extruded germlines, as in (3D-E). (G) Untethered Y479A. (H) Tethered Y479A. (I) Quantitation of tethered vs untethered Y479A. P-values as in (F). (J) Western blots after FBF-2 and NTL-1 co-immunoprecipitation. Left, input lysates (1%); right, FLAG IP (10%). NTL-1:V5 co-immunoprecipitates with both wild-type and Y479A.

Journal: Developmental cell

Article Title: PUF partner interactions at a conserved interface shape the RNA binding landscape and cell fate in Caenorhabditis elegans

doi: 10.1016/j.devcel.2024.01.005

Figure Lengend Snippet: (A) Tethering assay. FBF-2 is tethered by λN22 binding to boxB hairpins in the reporter RNA. Modified from Aoki et al. 201891. (B) Above, location of gonadal arm (black box) within animal. Below, location of distal region (dotted box) within gonadal arm. Asterisk marks distal end. Germline stem cells (GSCs) reside at the most distal end of germline and their daughters begin differentiation as they move proximally. (C) Diagram of distal gonad, showing distance from the distal end in microns below and extents of abundant FBF-2 (black line) and lower FBF-2 (dotted line) above. (D-E) Representative z-projections of extruded germlines. 20μm scale bar in (D) applies to all images. Dotted line marks gonad boundary; asterisk marks distal end. Top, GFP reporter expression (green); bottom, FBF-2FLAG staining (magenta). (D) Untethered wild-type FBF-2. (E) Tethered wild-type FBF-2. (F) ImageJ quantitation of reporter signal from tethered vs untethered FBF-2. GFP abundance plotted against distance from distal end. Solid line shows mean abundance and shading shows 95% confidence interval. Each plot represents three biological replicates with at least 10 gonad arms per replicate. P-values are given for pooled data in 0-35, 35-70 and 70-100 μm regions (black bars). P-values: *** p < 0.001, ** p < 0.01, * p < 0.05, ns (not significant) p > 0.05. Exact p-values in Table S4. (G-H) Representative z-projections of extruded germlines, as in (3D-E). (G) Untethered Y479A. (H) Tethered Y479A. (I) Quantitation of tethered vs untethered Y479A. P-values as in (F). (J) Western blots after FBF-2 and NTL-1 co-immunoprecipitation. Left, input lysates (1%); right, FLAG IP (10%). NTL-1:V5 co-immunoprecipitates with both wild-type and Y479A.

Article Snippet: mouse monoclonal αV5 , Bio-Rad , Cat# MCA1360, RRID:AB_322378.

Techniques: Binding Assay, Modification, Expressing, Staining, Quantitation Assay, Western Blot, Immunoprecipitation

KEY RESOURCES TABLE

Journal: Developmental cell

Article Title: PUF partner interactions at a conserved interface shape the RNA binding landscape and cell fate in Caenorhabditis elegans

doi: 10.1016/j.devcel.2024.01.005

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: mouse monoclonal αV5 , Bio-Rad , Cat# MCA1360, RRID:AB_322378.

Techniques: Virus, Recombinant, Protease Inhibitor, Software, Sequencing

(A) FBF-1 and FBF-2 are redundant for three germline roles. GSC, germline stem cell. (B) Adult gonad organization. Progenitor Zone (PZ, yellow) includes a pool of GSCs adjacent to the distal end (asterisk) and GSC daughters making the sperm to oocyte cell fate switch (s/o switch) more proximally; mature sperm (green) are in spermatheca and oocytes develop in proximal arm (magenta). The distal end is capped by the stem cell niche. (C-F) Representative z-projection images of extruded adult gonads; stained for sperm (α-SP56, green), oocytes (α-RME-2, magenta) and DNA (DAPI, cyan). Dotted line marks gonad boundary; asterisk marks distal end; yellow line marks PZ extent. 20μm scale bar in (C) applies to (C-F) (G) Gonad stained by smFISH for lst-1 RNA, a GSC marker (yellow). 20μm scale bar in (G) applies to (G-H). (H) Gonad stained with α-phospho-histone H3 (PH3) to visualize M-phase chromosomes (yellow) and DAPI (cyan). (I) Y479A-only germline phenotype. GSCs, germline stem cells maintained; s/o switch, sperm to oocyte switch occurs, n, number gonads scored. See also Figure S6.

Journal: Developmental cell

Article Title: PUF partner interactions at a conserved interface shape the RNA binding landscape and cell fate in Caenorhabditis elegans

doi: 10.1016/j.devcel.2024.01.005

Figure Lengend Snippet: (A) FBF-1 and FBF-2 are redundant for three germline roles. GSC, germline stem cell. (B) Adult gonad organization. Progenitor Zone (PZ, yellow) includes a pool of GSCs adjacent to the distal end (asterisk) and GSC daughters making the sperm to oocyte cell fate switch (s/o switch) more proximally; mature sperm (green) are in spermatheca and oocytes develop in proximal arm (magenta). The distal end is capped by the stem cell niche. (C-F) Representative z-projection images of extruded adult gonads; stained for sperm (α-SP56, green), oocytes (α-RME-2, magenta) and DNA (DAPI, cyan). Dotted line marks gonad boundary; asterisk marks distal end; yellow line marks PZ extent. 20μm scale bar in (C) applies to (C-F) (G) Gonad stained by smFISH for lst-1 RNA, a GSC marker (yellow). 20μm scale bar in (G) applies to (G-H). (H) Gonad stained with α-phospho-histone H3 (PH3) to visualize M-phase chromosomes (yellow) and DAPI (cyan). (I) Y479A-only germline phenotype. GSCs, germline stem cells maintained; s/o switch, sperm to oocyte switch occurs, n, number gonads scored. See also Figure S6.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse monoclonal αFLAG M2 Sigma-Aldrich Cat# F1804, RRID:AB_262044 mouse monoclonal αGFP 3E6 Thermo Fisher Scientific Cat# A-11120, RRID:AB_221568 mouse monoclonal αV5 Bio-Rad Cat# MCA1360, RRID:AB_322378 rabbit polyclonal αGLD-1 Betsy Goodwin (Jan et al., 1999) 87 N.A. mouse monoclonal αSP56 Sam Ward (Ward et al., 1986) 88 N.A. rabbit polyclonal αRME-2 Barth Grant (Grant and Hirsh, 1999) 89 N.A. mouse monoclonal αPH3 Cell Signaling Technology Cat# 9706, RRID:AB_331748 mouse monoclonal αGAPDH Proteintech Cat# 60004-1-Ig, RRID:AB_2107436 donkey polyclonal αMouse-Alexa647 Molecular Probes/Invitrogen Cat# A-31571, RRID:AB_162542 donkey polyclonal αRabbit-Alexa 488 Molecular Probes/Invitrogen Cat# A-21206, RRID:AB_2535792 goat polyclonal αMouse-HRP Jackson ImmunoResearch Labs Cat# 115-035-003, RRID:AB_10015289 Bacterial and Virus Strains E. coli: OP50 Caenorhabditis Genetics Center (CGC) RRID:WB-STRAIN:WBStrain00041969 E. coli: OP50-1 (Streptomycin resistant OP50) Caenorhabditis Genetics Center (CGC) RRID:WB-STRAIN:WBStrain00041971 E. coli: DH5α Competent Cells Thermo Fisher Scientific Cat# 18265017 E. coli: BL21(DE3) Competent Cells - Novagen Millipore Sigma Cat# 69450 Chemicals, Peptides, and Recombinant Proteins Alt-R TM S.p.

Techniques: Staining, Marker

KEY RESOURCES TABLE

Journal: Developmental cell

Article Title: PUF partner interactions at a conserved interface shape the RNA binding landscape and cell fate in Caenorhabditis elegans

doi: 10.1016/j.devcel.2024.01.005

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse monoclonal αFLAG M2 Sigma-Aldrich Cat# F1804, RRID:AB_262044 mouse monoclonal αGFP 3E6 Thermo Fisher Scientific Cat# A-11120, RRID:AB_221568 mouse monoclonal αV5 Bio-Rad Cat# MCA1360, RRID:AB_322378 rabbit polyclonal αGLD-1 Betsy Goodwin (Jan et al., 1999) 87 N.A. mouse monoclonal αSP56 Sam Ward (Ward et al., 1986) 88 N.A. rabbit polyclonal αRME-2 Barth Grant (Grant and Hirsh, 1999) 89 N.A. mouse monoclonal αPH3 Cell Signaling Technology Cat# 9706, RRID:AB_331748 mouse monoclonal αGAPDH Proteintech Cat# 60004-1-Ig, RRID:AB_2107436 donkey polyclonal αMouse-Alexa647 Molecular Probes/Invitrogen Cat# A-31571, RRID:AB_162542 donkey polyclonal αRabbit-Alexa 488 Molecular Probes/Invitrogen Cat# A-21206, RRID:AB_2535792 goat polyclonal αMouse-HRP Jackson ImmunoResearch Labs Cat# 115-035-003, RRID:AB_10015289 Bacterial and Virus Strains E. coli: OP50 Caenorhabditis Genetics Center (CGC) RRID:WB-STRAIN:WBStrain00041969 E. coli: OP50-1 (Streptomycin resistant OP50) Caenorhabditis Genetics Center (CGC) RRID:WB-STRAIN:WBStrain00041971 E. coli: DH5α Competent Cells Thermo Fisher Scientific Cat# 18265017 E. coli: BL21(DE3) Competent Cells - Novagen Millipore Sigma Cat# 69450 Chemicals, Peptides, and Recombinant Proteins Alt-R TM S.p.

Techniques: Virus, Recombinant, Protease Inhibitor, Software, Sequencing